abstract：:Chlorambucil-induced apoptosis was assessed by three different flow cytometric methods in B-cell chronic lymphocytic leukaemia (B-CLL) cells cultured in vitro and the results were compared with those derived from the morphological assessment of the same samples. Spontaneous apoptosis was consistently observed in the c...

journal_title：Leukemia research

authors： Pepper C,Thomas A,Tucker H,Hoy T,Bentley P

更新日期：1998-05-01 00:00:00

abstract：:In this report we evaluated the exact expression pattern of c-Kit on mobilized peripheral blood (PB) CD34+ cells. Using a monoclonal antibody against CD117 antigen (95C3), flow cytometric analysis revealed that approximately 25% of the mobilized PB CD34+ cells coexpress c-Kit. This cell fraction showed a considerable ...

journal_title：Leukemia research

authors： Kuçi S,Taylor G,Neu S,Schumm M,Niethammer D,Handgretinger R

更新日期：1998-04-01 00:00:00

abstract：:The expression of various proto-oncogenes in primary culture of lymphocytes from peripheral blood of bovine with chronic lymphocytic leukemia (CLL) was studied. Cellular proto-oncogenes encode proteins that propagate growth, differentiation or apoptosis signals from cell membrane to nucleus. The proliferation and diff...

journal_title：Leukemia research

authors： Kalvelyte AV,Pabrezaite LC

更新日期：1998-02-01 00:00:00

abstract：:We have isolated an M-CSF-like membrane-associated growth factor from human leukemic J6-1 cells that can enhance the growth and colony formation of J6-1 cells in vitro. Indirect evidence suggests that this membrane-associated M-CSF-like growth factor may do so by stimulating a corresponding receptor co-expressed on th...

journal_title：Leukemia research

authors： Wu KF,Rao Q,Zheng GG,He ZH,Ying HG,Song YH,Chen BD

更新日期：1998-01-01 00:00:00

abstract：:The human lymphoblastoid leukemic cell line (CCRF-CEM) was induced to differentiate with phorbol 12-myristate 13-acetate (PMA). During differentiation, assessed by monitoring the cluster of differentiation (CD) profile, the prosome (proteasomes, multi-catalytic proteinase) distribution and composition were studied by ...

journal_title：Leukemia research

authors： Baz A,Henry L,Chateau MT,Scherrer K,Bureau JP

更新日期：1997-11-01 00:00:00

abstract：:Hybrid (C56BL/6 x DBA) (BDF1; H-2b/H-2d) mice bearing the P815 leukemia (H-2d) were grafted with a (CBA x C57BL/6)F1 (CBF1; H-2k/H-2b) cell suspension, comprising bone marrow cells (BMC; 25 x 10(6)/mouse) and spleen cells (SC; 55 x 10(6)/mouse) on day-4, then treated with cyclophosphamide (200 mg/kg) on day-2 and fina...

journal_title：Leukemia research

authors： Seledtsov VI,Seledtsova GV,Avdeev EV,Samarin DM,Kozlov VA

更新日期：1997-10-01 00:00:00

abstract：:AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stage...

journal_title：Leukemia research

authors： Zhang X,Vik TA

更新日期：1997-09-01 00:00:00

abstract：:Endonucleases capable of producing internucleosomal DNA cleavage are one of the key enzymes in apoptosis. We examined endonuclease activities contained in nuclei of CD34+ and erythroid cells in the bone marrow (BM) from 12 patients with the myelodysplastic syndromes. The levels of Mg(2+)-dependent and acidic endonucle...

journal_title：Leukemia research

authors： Anzai N,Kawabata H,Hishita T,Yoshida Y,Ueda Y,Okuma M

更新日期：1997-08-01 00:00:00

abstract：:In 141 adult patients with diagnosis of acute myeloid leukemia the overall expression and intensity of expression of CD34 antigen on leukemic cells was investigated. Myeloid blasts were tested by applying direct immunofluorescence staining using anti-CD34 fluorescein monoclonal antibody in flow cytometry. CD34 antigen...

journal_title：Leukemia research

authors： Raspadori D,Lauria F,Ventura MA,Rondelli D,Visani G,de Vivo A,Tura S

更新日期：1997-07-01 00:00:00

abstract：:Treatment failure of patients with acute myelogenous leukemia (AML) is frequently due to the development of multidrug resistance phenotype blasts. We have expressed a fusion protein consisting of human granulocyte-macrophage colony stimulating factor (GMCSF) fused to the N-terminus of a lectin-deficient ricin toxin B ...

journal_title：Leukemia research

authors： Burbage C,Tagge EP,Harris B,Hall P,Fu T,Willingham MC,Frankel AE

更新日期：1997-07-01 00:00:00

abstract：:Microtubular reorganisation contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic drugs [1-3]. The aim of this study was to correlate the changes in the microtubules (MTs) with behavior of the centrosome in apoptotic cells, and to see whether post-translat...

journal_title：Leukemia research

authors： Pittman S,Geyp M,Fraser M,Ellem K,Peaston A,Ireland C

更新日期：1997-06-01 00:00:00

abstract：:MONO-MAC-1 is a human cell line with properties of blood monocytes, which can be used as a model system to study monocytic functions in vitro. In the present study, we prepared a karyotype of MONO-MAC-1, analysed the growth behaviour, determined the presence of differentiation-associated antigens and studied the expre...

journal_title：Leukemia research

authors： Steube KG,Teepe D,Meyer C,Zaborski M,Drexler HG

更新日期：1997-04-01 00:00:00

abstract：:A 53-year-old male patient was admitted to our hospital with painful splenomegaly. He was diagnosed as having chronic myelomonocytic leukemia (CMML) with leukocytosis, monocytosis, increased lysozyme concentrations in serum und urine, and lack of the Philadelphia chromosome. The clinical course of the disease was char...

journal_title：Leukemia research

authors： Aul C,Gattermann N,Germing U,Südhoff T,Hollmig KA,Heyll A

更新日期：1997-03-01 00:00:00

abstract：:Forty patients with high risk myelodysplastic syndrome (MDS) or acute myeloid leukemia (AML) preceded by MDS were treated with intensive induction and consolidation chemotherapy in a prospective multicenter pilot study. They were given two cycles of cytarabine 100 mg/m with 12-h intervals on days 1-7 and idarubicin 12...

journal_title：Leukemia research

authors： Ruutu T,Hänninen A,Järventie G,Koistinen P,Koivunen E,Kätkä K,Nousiainen T,Oksanen K,Pelliniemi TT,Remes K,Timonen T,Volin L,Elonen E

更新日期：1997-02-01 00:00:00

abstract：:Chronic lymphocytic leukemia (CLL) cells were cultured in a medium supplemented with 0.01-1 ng/ml interleukin-4 (IL-4) for 18 h, fixed and analyzed on a flow cytometer. The percentage of apoptotic (AP) cells with hypodiploid DNA content was determined from DNA histograms. IL-4 at 0.01 ng/ml protected from spontaneous ...

journal_title：Leukemia research

authors： Frankfurt OS,Byrnes JJ,Villa L

更新日期：1997-01-01 00:00:00

abstract：:We have previously shown that HL-60 cells exposed to all-trans retinoic acid (ATRA) after treatment with a non-cytotoxic concentration of vincristine (VCR) result in granulocytic maturation and differentiation, suggesting that VCR might exhibit a synergistic action with ATRA in the treatment of acute promyelocytic leu...

journal_title：Leukemia research

authors： Leung M,Wong K

更新日期：1997-01-01 00:00:00

abstract：:The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos...

journal_title：Leukemia research

authors： Ma DD,Wei AQ

更新日期：1996-11-01 00:00:00

abstract：:The thymic stroma has long been implicated in AKR thymic leukaemia. In this study an extensive panel of monoclonal antibodies was used to investigate changes in the AKR thymic microenvironment, in parallel with thymocyte differentiation of normal (2 month), preleukaemic (5-7 month) and leukaemic (> 7 month) mice. We f...

journal_title：Leukemia research

authors： Davey GM,Tucek-Szabo CL,Boyd RL

更新日期：1996-10-01 00:00:00

abstract：:Aplastic anemia, myelodysplastic syndromes (MDS) and chronic myeloproliferative diseases (MPD) are stem cell disorders. There is no clear-cut demarcation of them. Hypoplastic MDS displays features of aplastic anemia and MDS, on the other side mixed myelodysplastic and myeloproliferative syndromes (MDS-MPS) develop. In...

journal_title：Leukemia research

authors： Neuwirtová R,Mociková K,Musilová J,Jelínek J,Havlícek F,Michalová K,Adamkov M

更新日期：1996-09-01 00:00:00

abstract：:The incidence of B cell chronic lymphocytic leukemia (B-CLL) is lower in Asian countries, including India, when compared with Western countries. The bcl-2 gene rearrangement was restricted to the 5' region and seen in only one of the 20 Indian patients analyzed. The incidence remains similar to that reported from West...

journal_title：Leukemia research

authors： Parikh PM,Desai S,Naresh KN,Shakuntaka B,Laxminarayan K,Nair CN,Ladanyi M,Soman CS,Advani SH,Chaganti RS

更新日期：1996-09-01 00:00:00

abstract：:Retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (D3) are well known for inducing differentiation in many leukemic cell lines. The nuclear signalling pathways of RA and D3 are mediated through their cognate receptors, the retinoic acid receptor (RAR) and vitamin D3 receptor (VDR), respectively. Retinoid X receptor (RXR...

journal_title：Leukemia research

authors： Nakajima H,Kizaki M,Ueno H,Muto A,Takayama N,Matsushita H,Sonoda A,Ikeda Y

更新日期：1996-08-01 00:00:00

abstract：:In order to establish diagnostic criteria for hypocellular acute leukemia (HL), we have reviewed 32 cases selected on the basis of hypothetical 40% or less cellularity, by focusing on morphology, immunophenotype, karyotype and response to low dose Ara-C (LDAC) regimen and compared them with 40 cases of myelodysplastic...

journal_title：Leukemia research

authors： Nagai K,Kohno T,Chen YX,Tsushima H,Mori H,Nakamura H,Jinnai I,Matsuo T,Kuriyama K,Tomonaga M,Bennett JM

更新日期：1996-07-01 00:00:00

abstract：:Twenty-three patients with acute myelogenous leukemia (AML) in first relapse were treated with high-dose cytosine-arabinoside (Ara-C) and amsacrine or idarubicin. To prime the cells, the patients were given rhGM-CSF. We studied the influence of 48-h infusion of rhGM-CSF on proliferation and Ara-C sensitivity of leukem...

journal_title：Leukemia research

authors： Lacombe F,Puntous M,Dumain P,Cony-Makhoul P,Belloc F,Bernard P,Boisseau M,Reiffers J

更新日期：1996-06-01 00:00:00

abstract：:We have utilized the MTT assay to measure the metabolic activity of cells from the bone marrow of 55 patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) and non-clonal disease. Doubling dilutions of cells were exposed to MTT for 3-4 h. The mean optical density of the formazan produced by each c...

journal_title：Leukemia research

authors： Elgie AW,Sargent JM,Taylor CG,Williamson C

更新日期：1996-05-01 00:00:00

abstract：:We investigated the possible influence of recombinant (r) sIL-6R on the growth of three IL-6 non-responsive or weakly IL-6 responsive long-term myeloma cell lines. The three cell lines chosen for the study (U266, L363 and Fravel) all expressed gp130 but differed in their expression of IL-6R and IL-6. mRNA analysis by ...

journal_title：Leukemia research

authors： Diamant M,Hansen MB,Rieneck K,Svenson M,Yasukawa K,Bendtzen K

更新日期：1996-04-01 00:00:00

abstract：:The effect of VM-26, a topoisomerase II targeting drug, on IW32 murine erythroleukemia cells was investigated. The VM-26 induced IW32 cells to differentiate at a non-toxic but cytostatic concentration (0.01 microgram/ml). More than 40% of the cells were induced to synthesize hemoglobin, and cells were arrested in G2/M...

journal_title：Leukemia research

authors： Tan SK,Tang SJ,Wang MC,Hwang J,Wang FF

更新日期：1996-03-01 00:00:00

abstract：:In a previous study we reported that a new anthracycline derivative (moflomycin) exhibited a higher antileukemic activity compared to other anthracyclines, such as daunorubicin and doxorubicin. To explain the superior antileukemic effect of moflomycin and to disclose a possible structure-activity relationship, we inve...

journal_title：Leukemia research

authors： Andrivon W,Saucier JM,Auclair C,Monneret C,Florent JC,Guillosson JJ,Nafziger J

更新日期：1996-02-01 00:00:00

abstract：:The genes for the CDK4/6-inhibitors p16INK4A/MTS1 and p15INK4B/MTS2 are frequently deleted in hematological malignancies. A new member of this family of CDK4/6 inhibitors is p18. In order to assess p18 growth-suppressor gene alterations in hematological neoplasms, we investigated 31 lymphoma and leukemia cell lines by...

journal_title：Leukemia research

authors： Siebert R,Willers CP,Fosså A,Kloke O,Nowrousian MR,Seeber S,Opalka B

更新日期：1996-02-01 00:00:00

abstract：:The levels of expression of the activation antigen CD98 were studied by mAB CAF7 in 51 newly diagnosed consecutive cases of childhood acute lymphoblastic leukemia aged from 1 to 13 years. The mean follow-up was 8 months. A wide range of CAF7 expression was observed, the highest mean fluorescence intensity exceeding th...

journal_title：Leukemia research

authors： Taskov H,Pashov A,ffmitrova E,Yordanova M,Serbinova M

更新日期：1996-01-01 00:00:00

abstract：:A patient with myelodysplastic syndromes (MDS) developed eosinophilia during treatment with recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF). To study the mechanism of this eosinophilia, we investigated the proliferation of eosinophil colony-forming units (CFU-Eo) in nine patients and four...

journal_title：Leukemia research

authors： Koike M,Ishiyama T,Yokoyama A,Kawakami K,Nakamaki T,Tomoyasu S,Tsuruoka N

更新日期：1995-12-01 00:00:00

abstract：:Helix-loop-helix (HLH) transcription factors are involved in cellular growth and differentiation. The Id (inhibitor of DNA binding and differentiation) HLH proteins, in a dominantly negative fashion, regulate transcriptional activities of basic HLH proteins. We examined by northern hybridization the expression of Id2 ...

journal_title：Leukemia research

authors： Ishiguro A,Spirin K,Shiohara M,Tobler A,Norton JD,Rigolet M,Shimbo T,Koeffler HP

更新日期：1995-12-01 00:00:00

abstract：:Thermal stress induces expression of a family of heat shock proteins which may regulate the synthesis of various cellular genes. We investigated the effect of heat shock on polyadenylation in Epstein-Barr Virus (EBV) negative and EBV transformed human Burkitt's lymphoma (BL) B-cell lines. Incubation of the BL B-cell l...

journal_title：Leukemia research

authors： Kumar A,Kurl RN,Kryworuchko M,Diaz-Mitoma F,Sharma S

更新日期：1995-11-01 00:00:00

abstract：:A diagnosis of myelodysplastic syndrome was made in an 18-year-old patient with Warkany syndrome due to constitutional trisomy 8 mosaicism. The possible causal role of this particular chromosome constitution with respect to myelodysplasia and embryonal childhood tumors is discussed. ...

journal_title：Leukemia research

authors： Zollino M,Genuardi M,Bajer J,Tornesello A,Mastrangelo S,Zampino G,Mastrangelo R,Neri G

更新日期：1995-10-01 00:00:00

abstract：:A national cooperative group trial was conducted in 153 patients with chronic myelogenous leukemia (CML) in chronic phase treated with oral pulse busulfan to determine if oral vitamin A can increase the time to blast crisis and enhance survival of patients. Patients diagnosed within 1 year and in the chronic phase of ...

journal_title：Leukemia research

authors： Meyskens FL Jr,Kopecky KJ,Appelbaum FR,Balcerzak SP,Samlowski W,Hynes H

更新日期：1995-09-01 00:00:00

abstract：:We have previously reported that serum stem factor (SCF) levels are significantly lower in patients with myelodysplastic syndrome (MDS) than normal controls. We have now studied the effects of adding SCF to cultures of blood mononuclear cells from patients with MDS and normal subjects. Three of 17 patients with MDS sh...

journal_title：Leukemia research

authors： Masters GS,Baines P,Tang C,Bowen D,Burnett AK

更新日期：1995-08-01 00:00:00

abstract：:Although verapamil, cyclosporin A. cremophor EL and PSC-833 are active as multidrug resistance modulators, there has been limited study of these compounds as possible chemotherapy enhancing agents against drug-sensitive tumors. We compared these agents as modifiers of VP-16 cytotoxicity in vitro and modifiers of VP-16...

journal_title：Leukemia research

authors： Slater L,Sweet P,Wetzel M,Stupecky M,Osann K

更新日期：1995-08-01 00:00:00

abstract：:Interleukin-8 (IL-8) mRNA was rapidly, but not permanently, induced at high levels by phorbol-12myristate-13acetate (PMA) in HL60 cells. Ongoing protein synthase does not seem to be required for the initial induction of IL-8 gene expression. However, the rate of transient induction kinetics was modulated by cyclohexim...

journal_title：Leukemia research

authors： Meier RW,Niklaus G,Fey MF,Tobler A

更新日期：1995-07-01 00:00:00

abstract：:A retrospective cytogenetic study was performed to determine whether non-random chromosome aberrations were related to the outcome of marrow transplantation for advanced stage acute leukemia (AL) and chronic myelogenous leukemia (CML). The patients were registered on SWOG-8612, a randomized comparison of busulphan and...

journal_title：Leukemia research

authors： Slovak ML,Kopecky KJ,Wolman SR,Henslee-Downey JP,Appelbaum FR,Forman SJ,Blume KG

更新日期：1995-06-01 00:00:00

abstract：:240 patients with MDS studied cytogenetically at diagnosis between 1981 and 1990 were followed until death or until April 1992 to evaluate the prognostic significance of FAB classification, age and karyotype. 61 patients (25.4%) subsequently transformed into AML and 176 (73.3%) died during the follow-up period. Patien...

journal_title：Leukemia research

authors： Musilová J,Michalová K,Zemanová Z,Neuwirthová R,Dohnalová A

更新日期：1995-05-01 00:00:00

abstract：:Using the human erythroleukaemic cell line K562 cl.6 and its daunorubicin-resistant subline K/DAU600, and the human T-lymphoblastic leukaemic cell line CCRF-CEM and its vinblastine-resistant subline CEM/VLB100, we have shown that the drug-resistant cell lines were more sensitive to cytotoxicity induced by tumour necro...

journal_title：Leukemia research

authors： Jia L,Jiang XR,Razak K,Wu YL,Newland AC,Kelsey SM

更新日期：1995-03-01 00:00:00